Formulation, Development and Evaluation of Epirubicine Loaded Liposomal Drug Delivery System

Abstract

Objective: The purpose of present study was to formulate Epirubicine Hcl liposomes. In the 
present study epirubicin Hcl liposomes are formulated using various biolipids and Stabilizers 
(Positive and Negative) to check effect of drug loading and particle size
Methods: The preparation of liposomes with Soybean lecithin was prepared by dried thin film 
hydration technique using rotary evaporator. Accurately weighed quantities of Soy lecithin, 
cholesterol, Stearyl amine and Dicetylphosphate are dissolved in chloroform and rotated in a 
rota-vap by applying vacuum of about 25mmHg at 25°C, until it forms a thin film.
Results: The Morphology and surface appearance of Liposomes were examined by using SEM. 
The SEM photographs of F2 and F6 formulation showed that the particles have smooth surface. 
The assay value is determined for all the formulations from F1to F6. The assay value is within 
the limit (90%) for all the formulations.
Conclusion: From the executed experimental results, it could be concluded that the stabilizers 
like Stearylamine and Dicetylphosphate along with Soy lecithin and cholesterol were suitable 
carrier for the preparation of epirubicin Liposomes.